Slot Blot Hybridization - an overview

Slot Blot Hybridization - an overview slot blot apparatus Add 200 ul of TBS to 48 chambers in the apparatus Apply the low-speed vacuum to filter the liquid through the membrane Add 200 ul of the fibril samples, the slot machine winner Use the Bio-Dot SF Microfiltration System for easy, reproducible binding of proteins or nucleic acids in solution onto membranes in a 48-well slot format

slot machine winner nylon membranes using a slot blot apparatus The membranes were baked, hybridized to the AAV probe, washed, and autoradiographed at –70 C for 90 min The

slot wd Presoak 3 blotting papers and 1 nitrocellulose membrane in 6x SSC for 10 min · Clamp apparatus closed using the two Assemble the dot blot apparatus with a pre-wetted nylon membrane Load denatured viral DNA and linearized standard plasmid DNA solutions into the wells